In order to find out biochemical markers for adenocarcinoma of stomach, nucleic acid and protein contents, activities of deoxyribonuclease(DNase), ribonuclease(RNase), 5'-nucleotidase and amylase were determined in gastric juice and stomach
cancer
tissues of patients with gastric cancer. Also analysed were the enzymes and proteins isolated from gastric juice and stomach cancer tissue by a DEAE-cellulose column chromaography to investigate the specific tumor markers for stomach cancer .
concetrations of DNA and protein were significantly increased in the stomach cancer tissue, but RNA content was unchanged. Activities of acid DNase and 5'-nucleotidase were significantly increased was significantly decreased in stomach cancer
tissue,
but increased in gastric juice of patients with stomach tissue and gastric juice, was stomach cancer.
Neutral RNase and acid DNase were isolated from stomach cancer tissue by a DEAE-cellulose column chromatogrqaphy into single peak each, suggesting the absence of the RNase and DNase isozymes specific to the cancer is the stomach cancer tissue.
Two
peaks
each for neutral RNase and amylase isozymes were isolated by a DEAE-cellulose column chromatography from gastric juice of patients with stomach cancer.
The present study indicated that DNA and protein contents and activities of acid DNase, neutral 5'-nucleotidase and amylase in stomach tissue, and activities of neutral RNase and amylase in gastric juice could be used for biochemical markers for
stomach
cancer. Also indicated was the use as specific tumor markers of RNase and amylase isozyme isolated from gastric juice of patients with stomach cancer. The appropriate combination of to or more tumor markers might improve the specificity for the
detection of stomach cancer.
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